C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/44 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01)
Patent
CA 2171327
A method is provided for producing adipic acid. The method comprises the steps of culturing a cell transformant characterized by the constitutive expression of structural genes encoding 3-dehydroshikimate dehydratase, protocatechuate decarboxylase, and catechol 1,2-dioxygenase for a period of time sufficient to convert a carbon source to cis, cis-muconic acid and hydrogenating the cis, cis-muconic acid to produce adipic acid.
La présente invention concerne un procédé de production d'acide adipique. Le procédé comporte plusieurs opérations. La première est la culture d'un transformant de cellule, caractérisée par l'expression constitutive de gènes structuraux codant la 3-déshydroshikimate déhydratase, la protocatéchuate décarboxylase et la catéchol 1,2-dioxygénase. Cette première phase dure suffisamment longtemps pour convertir une source carbonée en acide cis, cis-muconique. La seconde phase est l'hydrogénation de l'acide cis, cis-muconique pour produire l'acide adipique.
Draths Karen M.
Frost John W.
Purdue Research Foundation
Smart & Biggar
LandOfFree
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