C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 21/02 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2100788
2100788 9213088 PCTABS00014 A method for preparing a ds DNA from a ss DNA template, which method includes: (a) providing a first DNA strand; (b) adding a homopolymeric oligonucleotide tail to the 3' end of the first DNA strand, to yield a tailed first DNA strand; (c) providing a ss homopolymeric oligonucleotide primer complementary to a portion of the tail; (d) contacting the primer with the tailed first DNA strand; (e) synthesizing, in the presence of the primer and the tailed first DNA strand, a second DNA strand complementary to the first DNA strand; and (f) removing the tail and the primer from the first and second DNA strands, respectively; provided that one or both of the tail and the primer contain(s) RNA.
Fukuoka Shin-Ichi
Scheele George
Beth Israel Hospital Association
Kirby Eades Gale Baker
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