Synthesis of oligonucleotides

Details Synthesis of oligonucleotides Synthesis of oligonucleotides

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2289204

A method of preparing an immobilized oligonucleotide having a free 3'-end comprises the steps of: i) preparing an oligonucleotide attached in a first position to a solid support via its 3'-end and having a free 5'-end; ii) binding said oligonucleotide in a second position remote from the 3'-end to the solid support; and iii) selectively releasing the 3'-end of the oligonucleotide from the solid support to obtain the oligonucleotide attached to the support in said second position in a reversed orientation with a free 3'-end.

L'invention concerne un procédé de préparation d'un oligonucléotide immobilisé ayant une terminaison 3' libre. Ce procédé consiste à (i) préparer un oligonucléotide fixé par sa terminaison 3' dans une première position à un support solide et ayant une terminaison 5' libre; (ii) lier cet oligonucléotide au support solide dans une seconde position à une certaine distance de la terminaison 3'; et (iii) détacher sélectivement la terminaison 3' de l'oligonucléotide du support solide pour obtenir un oligonucléotide fixé au support dans la seconde position, avec une orientation inversée et une terminaison 3' libre.

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