Synthesis of stereospecific oligonucleotide phosphorothioates

Details Synthesis of stereospecific oligonucleotide phosphorothioates Synthesis of stereospecific oligonucleotide phosphorothioates

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/04 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2208528

Disclosed is a method for synthesizing of stereospecific (Rp) phosphorothioate oligonucleotides. In this method, a primer comprising a plurality of deoxyribonucleotides and a ribonucleotide at the 5' terminal or 5' penultimate position, is annealed to a template. The structure is contacted with a mixture of deoxynucleoside .alpha.-triphosphate Sp diastereomers and a DNA polymerase to form a PS-Rp oligodeoxynucleotide extension which is liberated as a single- stranded PS-Rp oligonucleotide by cleavage after the ribonucleotide in the primer. Also disclosed are PS-Rp oligonucleotides and oligonucleotides prepared according to this method.

L'invention concerne un procédé de synthèse d'oligonucléotides de phosphorothioates stéréospécifiques (Rp). Ce procédé consiste à hybrider à une matrice une amorce composée d'une pluralité de désoxyribonucléotides et d'un ribonucléotide au niveau de la position terminale 5' ou de la position pénultième 5'. Cette structure est mise en contact avec un mélange de diastéréomères Sp de .alpha.-triphosphate de désoxynucléosides et d'une ADN polymérase afin qu'une extension d'oligodeoxynucléotides PS-Rp soit formée et libérée en tant qu'oligonucléotide monocaténaire PS-Rp par coupure après le ribonucléotide de l'amorce. L'invention concerne également des oligonucléotides PS-Rp, ainsi que des oligonucléotides préparés selon ce procédé.

Affiliated with

Also associated with

Rating

Synthesis of stereospecific oligonucleotide phosphorothioates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Synthesis of stereospecific oligonucleotide phosphorothioates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Synthesis of stereospecific oligonucleotide phosphorothioates will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2057760