C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.22, 195/1.
C12N 15/00 (2006.01) C07H 21/00 (2006.01) C12N 1/20 (2006.01) C12P 21/00 (2006.01)
Patent
CA 1201668
ABSTRACT OF THE DISCLOSURE The Specification discloses: 1. Recombinant microbial cloning vehicles comprising heterologous DNA coding for the expression of mammalian hor- mone (e.g., somatostatin) and other polypeptides, including plasmids suited for the transformation of bacterial hosts. The latter incorporate a regulon homologous to the host in its untransformed state, in reading phase with the structural gene for the heterologous DNA; 2. Cloning vehicles coding for the microbial expres- sion of a protein variously comprising a desired polypeptide product and additional protein from which the desired product may be cleaved; and 3. Methods of preparing synthetic structural genes coding for the expression of mammalian polypeptides in micro- bial cloning systems.
315820
Genentech Inc.
Gowling Lafleur Henderson Llp
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