Synthetic gene encoding human epidermal growth factor 2/neu...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2532460

Synthetic polynucleotides encoding human HER2/neu or a truncated form thereof, are provided, the synthetic polynucleotides being codon-optimized for expression in a human cellular environment. The gene encoding hHER2 is commonly associated with the development of human carcinomas. The present invention provides compositions and methods to elicit or enhance immunity to the protein product expressed by the hHER2 tumor-associated antigen, wherein aberrant hHER2 expression is associated with a carcinoma or its development. This invention specifically provides adenoviral vector and plasmid constructs carrying codon-optimized human HER2 and codon-optimized truncated HER2, and discloses their use in vaccines and pharmaceutical compositions for preventing and treating cancer.

L'invention concerne des polynucléotides synthétiques codant le récepteur du facteur de croissance épidermique humain 2 (HER2)/neu ou une forme tronquée de celui-ci, lesdits polynucléotides présentant des codons optimisés aux fins d'expression dans un milieu cellulaire humain. Le gène codant hHER2 est généralement associé au développement de carcinomes humains. L'invention concerne des compositions et des procédés permettant d'éliciter ou d'améliorer l'immunité du produit protéique exprimé par l'antigène associé à la tumeur hHER2, l'expression aberrante du hHER2 étant associée à un carcinome ou au développement de celui-ci. L'invention concerne de manière spécifique un vecteur adénoviral et des produits de synthèse de plasmide porteurs du HER2 humain à codons optimisés et du HER2 tronqué à codons optimisés, ainsi que l'utilisation de ceux-ci dans des vaccins et des compositions pharmaceutiques, aux fins de prévention et de traitement du cancer.

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