Synthetic gene encoding rhesus monkey carcinoembryonic...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/12 (2006.01) A61K 39/00 (2006.01) A61K 48/00 (2006.01) C12N 15/85 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2534547

Synthetic polynucleotides encoding rhesus monkey carcinoembryonic antigen (CEA) are provided, the synthetic polynucleotides being codon-optimized for expression in a human cellular environment. The gene encoding CEA is commonly associated with the development of human carcinomas. The present invention provides compositions and methods to elicit or enhance immunity to the protein product expressed by the CEA tumor-associated antigen, wherein aberrant CEA expression is associated with a carcinoma or its development. This invention specifically provides adenoviral vector and plasmid constructs carrying codon- optimed rhesus monkey CEA and discloses their use in vaccines and pharmaceutical compositions for preventing and treating cancer.

L'invention concerne des polynucléotides de synthèse codant pour un antigène carcino-embryonnaire de singe rhésus (CEA), les polynucléotides de synthèse ayant des codons optimisés pour l'expression dans un environnement cellulaire humain. Le gène codant pour CEA est associé de façon commune au développement des carcinomes humains. L'invention concerne des compositions et des procédés pour activer ou améliorer l'immunité au produit protéique exprimé par l'antigène CEA associé à une tumeur, l'expression aberrante de CEA étant associée à un carcinome ou à son développement. L'invention concerne de façon spécifique un vecteur adénoviral et des plasmides hybrides portant le CEA de singe rhésus à codons optimisés, et se rapporte à leur utilisation dans des vaccins et des compositions pharmaceutiques pour prévenir et traiter le cancer.

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