Synthetic glucagon binding proteins

Details Synthetic glucagon binding proteins Synthetic glucagon binding proteins

C - Chemistry, Metallurgy

12

N

C12N 15/16 (2006.01) C07K 14/605 (2006.01) C07K 14/72 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2255541

A synthetic human glucagon (hGlu) binding protein designated hGlu.DELTA.252- 259 binding protein is cloned, expressed and used in an <u>in vitro</u> assay to screen for compounds that bind to the synthetic binding protein, including compounds that specifically stimulate or inhibit the binding of glucagon to the synthetic receptor. The invention includes the assay, the synthetic binding protein used in the assay, DNA encoding the synthetic binding protein, cells expressing the synthetic binding protein, and compounds identified through the use of the synthetic binding protein.

L'invention concerne une protéine synthétique de liaison au glucagon humain (hGlu), dénommées protéine de liaison à hGlu.DELTA.252-259, est clonée, exprimée et utilisée dans une analyse <u>in vitro</u> pour le dépistage de composés qui se lient à la protéine de liaison synthétique, y compris les composés qui stimulent ou inhibent spécifiquement la liaison du glucagon au récepteur synthétique. L'invention concerne l'analyse, la protéine de liaison synthétique utilisée dans l'analyse, l'ADN codant ladite protéine de liaison synthétique, des cellules exprimant ladite protéine et les composés identifiés au moyen de la protéine selon l'invention.

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