Synthetic hybrid plant promoter

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2304257

The present invention is directed to a synthetic hybrid promoter composed of polynucleotide segments derived from the E8 and E4 gene promoters. The hybrid promoter is capable of providing high-level expression of heterologous genes, particularly in transformed fruit. DNA constructs containing the E8-E4 hybrid promoter operably linked to an exemplary heterologous SAMase gene are effective in conferring a delayed ripening phenotype to transformed fruit.

La présente invention concerne un promoteur hybride synthétique composé de segments de polynucléotide dérivés de promoteurs de gènes E4 et E8. Le promoteur hybride est capable de fournir une expression de niveau élevé de gènes hétérologues, notamment dans un fruit transformé. Les constructions d'ADN contenant le promoteur hybride E8-E4, liées de manière fonctionnelle à un gène SAMase hétérologue exemplaire, sont efficaces pour attribuer un phénotype différé de mûrissement à un fruit transformé.

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