Synthetic ligation reassembly in directed evolution

C - Chemistry – Metallurgy – 12 – P

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Details

C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C40B 40/06 (2006.01) C40B 50/06 (2006.01) C40B 50/18 (2006.01) C12N 9/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2374667

The invention is directed to a process of preparing nucleic acid and poly nucleotide libraries by cloning non-randomly generated nucleotide fragments having ligatable ends into an expression vector. The expression of the polynucleotide provides an indirect means by which to screen the polynucleotide library.

Procédé de préparation de bibliothèques d'acide nucléique et de polynucléotides par clonage non aléatoire de fragments nucléotidiques produits dotés de terminaisons pouvant être ligaturées dans un vecteur d'expression. L'expression du polynucléotide fournit un moyen indirect qui permet de cribler la bibliothèque de polynucléotides.

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