Synthetic nucleic acids from aquatic species

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C07K 14/435 (2006.01) C12N 15/12 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2525582

A synthetic nucleic acid molecule is provided that includes nucleotides of a coding region for a fluorescent polypeptide having a codon composition differing at more than 25% of the codons from a parent nucleic acid sequence encoding a fluorescent polypeptide. The synthetic nucleic acid molecule has at least 3-fold fewer transcription regulatory sequences relative to the average number of such sequences in the parent nucleic acid sequence. The polypeptide encoded by the synthetic nucleic acid molecule preferably has at least 85% sequence identity to the polypeptide encoded by the parent nucleic acid sequence.

Cette molécule d'acide nucléique synthétique comprend les nucléotides d'une région de codage d'un polypeptide fluorescent ayant un codon d'une composition qui diffère de plus de 25 % de celle des codons d'une séquence parente d'acide nucléique de codage d'un polypeptide fluorescent. La molécule d'acide nucléique synthétique a au moins trois fois moins de séquences régulatrices de la transcription que le nombre moyen de ces séquences dans la séquence parente d'acide nucléique. Au moins 85 % des séquences du polypeptide codé par la molécule d'acide nucléique synthétique sont de préférence identiques à celles du polypeptide codé par la séquence parente d'acide nucléique.

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