Synthetic oplophorus luciferases with enhanced light output

C - Chemistry – Metallurgy – 12 – N

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C12N 9/02 (2006.01) C12Q 1/66 (2006.01)

Patent

CA 2758572

A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type Oplophorus luciferase and includes at least one amino acid substitution at a position corresponding to an amino acid in a wild-type Oplophorus luciferase of SEQ ID NO: 1. The modified luciferase polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the wild-type Oplophorus luciferase.

L'invention porte sur un polynucléotide codant pour un polypeptide de luciférase modifié. Le polypeptide de luciférase modifié a au moins 60 % d'identité de séquence d'acides aminés avec une luciférase d'Oplophorus de type sauvage et comprend au moins une substitution d'acide aminé en une position correspondant à un acide aminé dans une luciférase d'Oplophorus de type sauvage de SEQ ID NO : 1. Le polypeptide luciférase modifié a au moins l'une parmi une luminescence augmentée, une stabilité de signal augmentée, une stabilité de protéine augmentée par rapport à la luciférase d'Oplophorus de type sauvage.

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