G - Physics – 01 – N
Patent
G - Physics
01
N
73/93
G01N 30/90 (2006.01)
Patent
CA 1218875
SYSTEM AND APPARATUS FOR MULTI-DIMENSIONAL REAL-TIME CHROMATOGRAPHY ABSTRACT A column and detector are disclosed for two-dimensional column chromatography of a sample, in which the sample is injected close to a corner of a thin parallelepipedal column through which percolates a first solvent pumped at a con- trolled velocity. After a convenient time this flow is stopped and a stream of a second solvent is pumped at a controlled velocity in the perpendicular direction. Elution of the separated components is performed, the stream exiting from the column flows over a detector which monitors its composition at a large number of points, along the exit edge allowing the derivation of a two-dimensional chromatogram. The chromatographic bed can be honogeneous or made of a first portion including a first composition suitable to perform column chromatography in the first direction of the column and a second portion including a second composition suitable to perform column chromatography in a second direction of the column, wherein both compositions are selected from compositions available for column chroma- tography. A method for making a column and detector for three-dimensional chromatography is also disclosed. Methods for using such columns and detectors, over-pressure develop- ment of conventionally read two-dimensional thin column chromatograms, use of the thin-layer column as a multi- column chromatograph, injection of unknowns into the flowing solvent, optimum flow rates, and a special cuvette plate and associated apparatus for performing the methods are also disclosed.
457488
Beaver Lois A.
Guiochon Georges A.
Sim & Mcburney
Varex Corporation
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