System and apparatus for sequential processing of analytes

G - Physics – 01 – N

Patent

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G01N 21/75 (2006.01) B01J 10/00 (2006.01) B01J 19/00 (2006.01) B01L 3/00 (2006.01) C12Q 1/68 (2006.01) G01N 1/00 (2006.01) G01N 15/14 (2006.01) G01N 21/00 (2006.01) G01N 21/25 (2006.01) G01N 21/29 (2006.01) G01N 21/64 (2006.01) G06T 7/20 (2006.01) G11B 23/00 (2006.01) G01N 15/00 (2006.01)

Patent

CA 2291180

An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

La présente invention concerne un appareil et un système permettant d'analyser simultanément plusieurs analytes fixés dans des microparticules. Des microparticules possédant chacune une population uniforme composée d'un seul type d'analyte fixé dans celles-ci sont disposées de façon qu'elles forment un réseau plan sensiblement immobilisé à l'intérieur d'une chambre d'écoulement dans laquelle on procède aux différentes phases d'un processus d'analyse en distribuant aux microparticules une série de réactifs de traitement à l'aide d'un système fluidique commandé par un microprocesseur. En réponse aux phases de ce processus, un signal optique est généré à la surface de chaque microparticule, signal qui est caractéristique de l'interaction entre l'analyte porté par la microparticule et le réactif de traitement distribué. On analyse les différents analytes simultanément en collectant et en enregistrant des images des signaux optiques générés par toutes les microparticules du réseau plan. L'invention est caractérisée par la corrélation de la séquence des signaux optiques générés par chaque microparticule du réseau plan au cours du processus d'analyse.

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