System and method for analyzing antibiotic susceptibility of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/02 (2006.01) G01N 21/25 (2006.01) G01N 21/64 (2006.01) G01N 21/78 (2006.01) G01N 21/82 (2006.01) G01N 33/15 (2006.01) G01N 33/48 (2006.01) G01N 35/02 (2006.01)

Patent

CA 2349043

A system and method for analyzing samples, such as biological samples, to accurately and effectively determine the susceptibility of the samples to antimicrobial materials, so as to determine minimum inhibitory concentration (MIC) values for the respective samples and antimicrobial materials. At each of a plurality of time intervals, the system and method directs a plurality of different analyzing light wavelengths, such as red, green and blue wavelengths, onto each of a plurality of sample wells, and detects a respective resultant light wavelength emanating from the respective sample wells for each of the analyzing light wavelengths. The system and method uses resultant light wavelengths to generate at least two growth indicator characteristic curves representing, for example, the redox state and turbidity characteristics of the sample wells. The system then uses the redox state and turbidity characteristics of sample wells containing the same antimicrobial material to determine the MIC value for that material in relation to the sample contained in those wells.

L'invention porte sur un système et une méthode permettant d'analyser des échantillons, par exemple des échantillons biologiques, afin d'établir efficacement et de manière exacte la sensibilité des échantillons à des agents antimicrobiens, et de mesurer la concentration minimale inhibitrice (CMI) des échantillons et des agents antimicrobiens. € intervalle régulier et à plusieurs moments, le système, à l'aide de la méthode, envoie des longueurs d'onde différentes, comme des ondes rouges, vertes et bleues, une à la fois, dans un puit contenant un échantillon à la fois, et détecte respectivement chacune des longueurs d'onde émises. Le système et la méthode font appel aux longueurs d'ondes émises pour produire au moins deux courbes caractéristiques indicatrices de la croissance représentant, par exemple, l'état d'oxydoréduction ou la turbidité de chaque échantillon. Le système se sert alors de l'état d'oxydoréduction et de la turbidité des échantillons contenant le même agent antimicrobien pour déterminer la CMI propre à cet agent, dans un échantillon donné.

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