System and method for correcting primer extension errors in...

Details System and method for correcting primer extension errors in... System and method for correcting primer extension errors in...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2637617

An embodiment of method for correcting an error associated with phasic synchrony of sequence data generated from a population of substantially identical copie of a template molecule is described that comprises (a) detecting a signal generated in response to an incorporation of one or more nucleotides in a sequencing reaction; (b) generating a value for the signal; and (c) correcting the value for the phasic synchrony error using a first parameter and a second carry forward parameter.

L'invention concerne une méthode de correction d'une erreur associée à la synchronisation de phase des données de séquence générées à partir d'une population de copies sensiblement identiques d'une molécule modèle, qui comprend les étapes consistant à (a) détecter un signal généré en réaction à l'incorporation d'un ou de plusieurs nucléotides dans une réaction de séquençage; (b) générer une valeur pour le signal; et (c) corriger la valeur pour l'erreur de synchronisation de phase en utilisant un premier paramètre et un deuxième paramètre de report.

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