System and method for detection, identification and...

G - Physics – 01 – N

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G01N 9/30 (2006.01) G01N 1/22 (2006.01) G01N 1/34 (2006.01)

Patent

CA 2306032

A system (100) and method for detection, identification and monitoring of submicron sized particles. A gas or liquid sample is introduced into a collection stage (101) and passed to an extraction stage (102) for separation by density in a density gradient centrifuge (35). After extraction, the sample is passed to a purification and concentration stage (103) such as an ultrafiltration unit (21, 22) and finally to a detection stage (104), exemplified by an electrospray device (24), differential mobility analyzer (26) and condensation nucleus counter (27). The submicron sized particles to be detected include viruses and prions, which can be isolated and identified based on their physical characteristics without the use of biochemical reagents or assays.

Système (100) et procédé de détection, identification et surveillance de particules de taille inférieure au micron. Un échantillon gazeux ou liquide est introduit dans un étage de collecte (101) et transféré dans un étage d'extraction (102) en vue de la séparation par densité dans une centrifugeuse (35) en gradient de densité. Après extraction, l'échantillon est transféré dans un étage de purification et de concentration (103), tel qu'une unité d'ultrafiltration (21, 22), et finalement dans un étage de détection (104), par exemple un dispositif d'électropulvérisation (24), un analyseur de mobilité différentielle (26) et un compteur de noyaux de condensation (27). Les particules de taille inférieure au micron à détecter sont les virus et les prions, qui peuvent être isolés et identifiés sur la base de leurs caractéristiques physiques sans utilisation de réactifs biochimiques ou d'analyses.

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