System and method for differential protein expression and a...

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G01N 37/00 (2006.01) A61B 5/00 (2006.01) C12M 1/34 (2006.01) C12Q 1/68 (2006.01) G01N 24/00 (2006.01) G01N 27/62 (2006.01) G01N 33/00 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G06F 17/00 (2006.01) G06F 19/00 (2006.01) H01J 49/26 (2006.01)

Patent

CA 2453546

A cell protein profiling and diagnostic system is provided that fractionates (110) a protein content of a tissue sample into prot ein subgroups, independently performs mass spectroscopy (120) on each protein subgroup, creates a cell expression protein profile from the mass spectra, and identifies protein patterns associated with subject characteristics, such as biological conditions and diseases, based on the cell expression protein profile. In one embodiment, the protein patterns are identified with an artificial neural network, or other data mining or pattern recognition techniques.

L'invention concerne un système de profilage de protéines cellulaires et de diagnostic qui fractionne (110) le contenu protéique d'un échantillon de tissu en sous-groupes de protéines, qui effectue indépendamment une spectroscopie de masse (120) sur chaque sous-groupe de protéines, qui tire un profil de protéine d'expression cellulaire du spectre de masse et qui identifie des motifs protéiques associés aux caractéristique du sujet, telles que des conditions biologiques et des maladies, sur la base du profil de protéine d'expression cellulaire. Dans un mode de réalisation, les motifs protéiques sont identifiés au moyen d'un réseau neural artificiel ou d'autres techniques d'exploration de données ou de reconnaissance de motifs.

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