G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/60 (2006.01) A61K 51/00 (2006.01) C07C 331/16 (2006.01) G01N 30/72 (2006.01) G01N 33/483 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01) H01J 49/26 (2006.01) H01J 49/40 (2006.01)
Patent
CA 2553223
The present invention relates to modification of isotope ratios in peptides and polypeptides as an alternative to full isotopic exchange for coding in proteomics. Subtle modification of isotope ratio does not compromise protein identification experiments and can thus provide elemental composition data for accurate isotope ratio decoding. Application of subtle modification of isotope ratio proteomics (SMIRP) offers a convenient approach to in vivo isotope coding.
La présente invention a trait à la modification de rapports isotopiques dans des peptides et des polypeptides comme une alternative à l'échange isotopique complète pour le codage en protéomique. Une modification subtile du rapport isotopique ne compromet pas des expériences d'identification de protéines et peut ainsi fournir des données de composition élémentaire pour le décodage précis de rapports isotopiques. L'application d'une modification subtile de proétomique de rapports isotopiques offre une approche appropriée au codage isotopique in vivo.
Agus David B.
Katz Jonathan
Whitelegge Julian P.
Cedars-Sinai Medical Center
Oyen Wiggs Green & Mutala Llp
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