System and method for improved processing of nucleic acids...

C - Chemistry – Metallurgy – 07 – H

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C07H 21/00 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01) C12N 15/66 (2006.01) C40B 40/06 (2006.01)

Patent

CA 2716081

An embodiment of an adaptor element for efficient target processing is described that comprises a semi-comple-mentary double stranded nucleic acid adaptor comprising a non- complementary region and a complementary region, where the non-complementary region comprises a first amplification primer site and a second amplification primer site and the complemen-tary region comprises a sequencing primer site and one or more inosine species.

La présente invention concerne un mode de réalisation d'un élément adaptateur conçu pour traiter efficacement la cible et comprenant un adaptateur en acide nucléique à double brin semi-complémentaire, constitué d'une région non-complémentaire et d'une région complémentaire, la région non-complémentaire comprenant un premier site amorce d'amplification et un second site amorce d'amplification, la région complémentaire comprenant un site amorce de séquençage et au moins une espèce inosine.

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