System and method for producing synthetic microorganisms...

C - Chemistry – Metallurgy – 12 – P

Patent

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Details

C12P 21/00 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12N 15/01 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2702061

The disclosed invention relates to the generation of host cells containing rare codons and/or absent tRNAs, and the use of orthogonal tRNA systems that can insert a non-standard amino acid into a growing peptide chain. This invention combined with the capacity to synthesize whole genomes has important implications in synthetic biology, as it allows the rewriting of the genetic code of existing or newly designed organisms.

L'invention décrite concerne la génération de cellules hôtes contenant des codons rares et/ou des ARNt absents, et l'utilisation de systèmes d'ARNt orthogonaux qui peuvent incorporer un acide aminé non standard dans une chaîne peptidique en cours d'allongement. L'invention, combinée à la capacité de synthétiser des génomes entiers, a d'importantes implications en biologie synthétique, dans la mesure où elle permet de récrire le code génétique d'organismes existants ou nouvellement développés.

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