System and methods for electrophoretic separation of...

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G01N 27/447 (2006.01) G01N 27/453 (2006.01)

Patent

CA 2498354

Proteins can be rapidly separated to a high degree of resolution by electrophoresis on polymeric membranes that have high protein binding capacity. The electrophoretic separation is carried out in a low conductivity, water-miscible organic solvent buffer. The low conductivity of the organic solvent buffer minimizes heat generation, and the water-miscible nature of the organic solvent buffer permits the analysis of hydrophobic and low molecular weight proteins as well as hydrophilic proteins. When electrophoresis is conducted under non-denaturing conditions, it allows the detection of enzymatic activities, protein-protein interactions and protein~ligand interactions.

Il est possible de séparer rapidement des protéines avec un degré élevé de résolution en mettant en oeuvre une électrophorèse sur des membranes polymères dotées d'une capacité élevée de liaison aux protéines. La séparation électrophorétique est effectuée dans un tampon de solvant organique miscible avec l'eau de faible conductivité. La faible conductivité de ce tampon de solvant organique minimise la production de chaleur et le fait que le tampon de solvant organique soit miscible avec l'eau permet l'analyse de protéines hydrophobes et de faible poids moléculaire ainsi que de protéines hydrophiles. Lorsque l'électrophorèse est effectuée dans des conditions de non dénaturation, elle permet la détection des activités enzymatiques, des interactions protéine-protéine et des interactions protéine-ligand.

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