C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/63 (2006.01) C12N 15/09 (2006.01) C12N 15/64 (2006.01) C12N 15/79 (2006.01)
Patent
CA 2698627
The functional analysis of genes frequently requires the manipulation of large genomic regions. A yeast-bacteria shuttle vector is described that can be used to clone large regions of DNA by homologous recombination. Also described is a method for isolating entire genomes, including chloroplast genomes, or large portions thereof, and manipulating the same. Also described are methods for determining minimal genomes, minimal pathway requirements, and minimal organelle genomes.
L'analyse fonctionnelle des gènes requiert fréquemment la manipulation de grandes régions génomiques. L'invention porte sur un vecteur navette levure-bactérie qui peut être utilisé pour cloner de grandes régions de l'ADN par recombinaison homologue. L'invention se rapporte également à un procédé qui permet d'isoler des génomes entiers, y compris des génomes de chloroplastes, ou de grandes parties de ces derniers, et de les manipuler. L'invention concerne aussi des procédés qui permettent de déterminer des génomes minimaux, des exigences de voies minimales et des génomes d'organelles minimaux.
Mendez Mike
Mikkelson Kari
O'neill Bryan
Fetherstonhaugh & Co.
Sapphire Energy Inc.
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