System for diagnosing periodontal disease

Details System for diagnosing periodontal disease System for diagnosing periodontal disease

C - Chemistry, Metallurgy

12

Q

C12Q 1/37 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2078331

2078331 9114000 PCTABS00007 A colorimetric assay system for diagnosing periodontal disease utilizes a chromogenic test substance for measuring proteolytic activity in a specimen which may contain suspected periodontopathogenic bacteria. The colorimetric assay method of the present invention operates to detect the presence of proteolytic activity in a specimen of subgingival plaque, for example. A chromogenic test substance comprises a peptide substrate which is hydrolyzable by proteolytic enzymes in the plaque specimen to release the chromophore. Detection of a color change in the test substance indicates whether such proteolytic activity is present. In specific illustrative embodiment, the chromogenic test substance comprises N-benzoyl-DL-arginine-2-naphthylamide (BANA) or benzoyl-DL-arginine-p-nitroanilide (BAPNA). In the case of BANA, for example, the chromophore, .beta.-naphthylamide, is detected by the addition of a color developer, such as fast garnet. The development of a red-orange color is interpreted to indicate the presence of periodontal disease associated with anaerobic periodontopathogens, such as B. gingivalis), T. denticola, and B. forsythus, and the development of a yellow color is interpreted to indicate the absence of periodontal disease.

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