System for discovery of agents that block yersinia pestis...

Details System for discovery of agents that block yersinia pestis... System for discovery of agents that block yersinia pestis...

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C07K 14/21 (2006.01) C07K 14/24 (2006.01) C07K 16/40 (2006.01) C12N 9/12 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12Q 1/48 (2006.01) G01N 33/53 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2447386

Y. pestis and P. aeruginosa nucleic acid molecules encoding dnaE, holA, holB, holC, holD, holE, dnaX, dnaN, SSB, dnaG, dnaQ, proteins are provided. The encoded proteins are also provided. The nucleic acid molecules and proteins are useful for reconstituting replicases and polymerases for sequencing, amplification, and screening for compounds which modulate the function of the polyemersase or replicase.

L'invention concerne des molécules d'acide nucléique de Y. pestis et de P. aeruginosa codant les protéines dnaE, holA, holB, holC, holD, holE, dnaX, dnaN, SSB, dnaG, dnaQ. L'invention concerne également les protéines codées. Les molécules d'acide nucléique et les protéines sont utiles pour la reconstitution de réplicases et de polymérases destinées au séquençage, à l'amplification et au criblage de composés modulant la fonction de la polymérase ou de la réplicase.

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