System for electrophysiological measurements

G - Physics – 01 – N

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Details

G01N 33/483 (2006.01) G01N 27/416 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2424498

The present invention relates to a high through-put system for determining and/or monitoring electrophysiological properties of ion channels of ion channel-containing membranes, typically lipid membrane-containing structures such as cells. In particular, the invention provides a substrate which provides means for automatically positioning cells at measuring sites using electroosmotic flow in canals formed on or in the substrate. The electroosmotic flow is generated and controlled by electroosmotic flow pumps integrated on the substrate or positioned in relation thereto. Thereby, cells can be positioned in favourable measurement configuration at a plurality of sites for performing testing and measurements. Also, the invention relates to a main electric circuit for performing testing and measurements on a plurality of cells in parallel.

La présente invention concerne un système à haut rendement permettant de déterminer et/ou surveiller les propriétés électrophysiologiques des canaux ioniques de membranes contenant des canaux ioniques, en général des structures lipidiques contenant des membranes comme, par exemple, les cellules. L'invention concerne, en particulier, un substrat pourvu d'organes servant à positionner automatiquement des cellules sur des sites de mesure à l'aide d'un flux électroosmotique dans des canaux formés sur ou dans le substrat. Le flux électroosmotique est généré et régulé par des pompes de flux électroosmotique intégrées sur le substrat ou positionnées par rapport à celui-ci. Ainsi, les cellules peuvent être positionnées selon une configuration de mesure favorable sur plusieurs sites, permettant ainsi de procéder aux essais et aux mesures. L'invention concerne également un circuit électrique principal permettant de procéder aux essais et aux mesures sur plusieurs cellules en parallèle.

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