System for high-speed measurement and sorting of particles

Details System for high-speed measurement and sorting of particles System for high-speed measurement and sorting of particles

G - Physics

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N

G01N 15/10 (2006.01) B07C 5/00 (2006.01) B07C 5/342 (2006.01) G01N 15/00 (2006.01) G01N 15/14 (2006.01) G01N 21/25 (2006.01) G01N 21/47 (2006.01) G01N 21/84 (2006.01) G01N 33/483 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2106191

2106191 9216829 PCTABS00016 A system for sorting particles is arranged such that the presence or absence of multiple parameters (TOTAL, RARE1, RARE2, RARE3) is determined for each particle in a flow of particles, and a logic condition (R) is determined based on the multiple parameters. Thereafter, particles are outputted from the system when the logic condition (R) is present. Accordingly, multiparameter high-speed measurements of a rare subpopulation of particles (e.g. biological cells) can be made amidst a larger population of particles with differing characteristics. When attached to a multiparameter flow cytometer/cell sorter (10) and microcomputer, the system allows multiparameter analysis of cells at rates in excess of 100,000 cells/sec. This system can be an outboard module (12) attached to a commercially available or home-built flow cytometer.

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