System for in vitro transposition using modified tn5...

Details System for in vitro transposition using modified tn5... System for in vitro transposition using modified tn5...

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/22 (2006.01) C12N 15/85 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2265477

A system for in vitro transposition includes a donor DNA that includes a transposable element flanked by a pair of bacterial transposon Tn5 outside end repeat sequences, a target DNA into which the transposable element can transpose, and a modified Tn5 transposase having higher binding avidity to the outside end repeat sequences and being less likely to assume an inactive multimer form than wild type Tn5 transposase.

Un système de transposition in vitro comprend un ADN donneur contenant un élément transposable bordé d'une paire de transposons Tn5 bactériens à l'extérieur de séquences à répétitions terminales, un ADN cible dans lequel l'élément transposable peut se transposer, et une transposase Tn5 modifiée présentant une avidité de liaison supérieure à l'extérieur des séquences à répétitions terminales et moins susceptible de prendre une forme multimère inactive que la transposase Tn5 de type sauvage.

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