System for metering nutrient media to cell culture...

C - Chemistry – Metallurgy – 12 – M

Patent

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C12M 3/00 (2006.01) C12M 1/00 (2006.01) C12M 1/26 (2006.01)

Patent

CA 1270467

SYSTEM FOR METERING NUTRIENT MEDIA TO CELL CULTURE CONTAINERS AND METHOD ABSTRACT OF THE DISCLOSURE A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used from essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, scaled bag having polyethylene walls 0.013 inch thick.

536719

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