System for rate immunonephelometric analysis

Details System for rate immunonephelometric analysis System for rate immunonephelometric analysis

G - Physics

01

N

167/37, 73/59

G01N 21/51 (2006.01) G01N 21/27 (2006.01) G01N 21/47 (2006.01)

Patent

CA 1081497

ABSTRACT OF THE DISCLOSURE A system for the detection of precipitate-forming anti- gens by means of the reaction with their corresponding antibodies is disclosed. The reaction of precipitate-forming antigens with their respective antibodies produces a precipitate in proportion to either the antibody concentration or the antigen concentration, depending on which is present in excess. The method does not require formation of actual precipitate, but measure the forma- tion of "scattering centers" -- i.e., molecular aggregates of sufficient size to produce measurable scatter of light. The quantity of scattering centers formed is measured by nephelometric means and the measuring system performs and immunonephelometric analysis. In particular, the rate of change of the nephelometric signal with respect to time is measured and the peak rate and time to the peak rate established. From these simultaneous measurements both the antigen/antibody concentration and the condition of antibody or antigen excess are determined.

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