System for selective cell separation from cell concentrate

Details System for selective cell separation from cell concentrate System for selective cell separation from cell concentrate

C - Chemistry, Metallurgy

12

N

167/114, 17/26,

C12N 5/00 (2006.01) A61K 35/14 (2006.01) A61K 35/28 (2006.01) A61M 1/36 (2006.01) B03C 1/02 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 35/00 (2006.01) A61M 1/02 (2006.01)

Patent

CA 1335181

A system for the selective separation of a specific cel population from a heterogeneous cell mixture is disclosed herein. Means are provided for obtaining a selective cell concentrate from the heterogeneous cell mixture by separating the selective cell concentrate based upon the physical or biological properties of the concentrate. This may be done by centrifugation, if desired. A container is provided having particle means, the particle means being attached to a substance that is active for binding to a specific desired cell. One brings the cell concentrate into contact with the particle means within the container, for example, by providing sterile connection between the site where the cell concentrate is formed and the container. This permits incubation of the cell concentrate with the particle means, to thereby selectively bind a specific cell population from the cell concentrate to the particle means, creating a particle/cell conjugate. Magnetic means are then provided for separating the particle/cell conjugate from the remainder of the cell concentrate. A two-magnet system may be used to form the separation.

614111

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