C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/00 (2006.01) A01K 67/027 (2006.01) A61K 35/28 (2006.01) C07K 14/52 (2006.01) C12Q 1/70 (2006.01) A61K 48/00 (2006.01)
Patent
CA 2207216
The present invention relates to a method of engrafting human or non-human primate pluripotent stem cells, such as CD34+ cells, in a mouse. The method comprises administering to irradiated mice immortalized human or non-human primate bone marrow stromal cells which express cytokines supporting growth of cells, such as CD34+ cells, and, sequentially or simultaneously, administering purified human or non-human primate CD34+ cells. The stromal cells and CD34+ cells can be administered by intraperitoneal or intravenous injections. The invention can be used to evaluate gene therapy protocols for human CD34+ cells as well as having applications in the treatment of patients exposed to irradiation such as during a bone marrow transplant. The invention also provides an animal model for the study of human hematopoiesis.
La présente invention concerne un procédé permettant de favoriser la prise de greffe de cellules souches pluripotentes d'origine humaine ou provenant de primates, telles que des cellules CD34+, chez une souris. Ce procédé consiste à administrer, à des souris irradiées, des cellules du stroma de moelle osseuse d'origine humaine ou provenant de primates qui expriment des cytokines assurant la croissance des cellules, telles que des cellules CD34+, et à administer, de manière séquentielle ou simultanée des cellules CD34+ purifiées d'origine humaine ou provenant de primates. On peut administer les cellules du stroma et les cellules CD34+ par injections intraveineuses ou intrapéritonéales. Cette invention peut servir à évaluer des protocoles de thérapie génique pour les cellules CD34+ humaines et peut également avoir des applications relatives au traitement de patients soumis à une irradiation comme cela se produit lors d'une greffe de moelle osseuse. Cette inventin concerne également un modèle animal destiné à l'étude de l'hématopoïèse humaine.
Gartner Suzanne
Hall Eric
Kaushal Sumesh
Kessler Steven
La Russa Vince
Gartner Suzanne
Hall Eric
Kaushal Sumesh
Kessler Steven
Kirby Eades Gale Baker
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