System for transposing hyperactive recombinant derivatives...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/90 (2006.01) A61K 48/00 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2651413

The invention concerns a system for transposing a hyperactive recombinant derivative of Mos-1 transposon, comprising at least the two following partners: a) a Mos-1 pseudo-transposon in which an exogenous nucleotide sequence of interest replaces the nucleotide sequence encoding the original Mos-1 transposase; and b) a Mos-1 tranposase provided in trans in said pseudo-transposon, at least one of said partners being appropriately genetically modified to improve the transposition frequency of said exogenous nucleotide sequence of interest. Additionally to such systems, the invention concerns hyperactive Mos-1 transposons, hyperactive Mos-1 transposases, kits. The invention further concerns the use of one or more of abovementioned means for carrying out sequence transpositions, and more particularly efficient gene transfers.

La présente invention a pour objet un système de transposition recombinant hyperactif dérivé du transposon Mos-1, comprenant au moins les deux partenaires suivants : a) un pseudo-transposon Mos-1 dans lequel une séquence nucléotidique exogène d'intérêt remplace la séquence nucléotidique codant la transposase Mos-1 d'origine; et b) une transposase Mos-1 fournie en trans dudit pseudo-transposon, l'un au moins desdits partenaires étant génétiquement modifié de manière appropriée pour améliorer la fréquence de transposition de ladite séquence nucléotidique exogène d'intérêt. Outre de tels systèmes, l'invention fournit des pseudo-transposons Mos-1 hyperactifs, des transposases Mos-1 hyperactives, des kits. L'invention concerne également l'utilisation d'un ou plusieurs des moyens précédents pour réaliser des transpositions de séquences, et plus particulièrement des transferts de gènes, efficaces.

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