System, method and device for tissue-based diagnosis

G - Physics – 01 – N

Patent

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Details

G01N 33/487 (2006.01) A61B 10/02 (2006.01) A61M 27/00 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2752617

The current invention provides devices, methods and systems involving application of energy and/ or a liquefaction promoting medium to a tissue of interest to generate a liquefied sample comprising tissue constituents so as to provide for rapid tissue sampling, tissue decontamination as well as qualitative and/or quantitative detection of analytes that may be part of tissue constituents (e.g., several types of biomolecules, drugs, and microbes). In addition, the current invention provides specific compositions of the said liquefaction promoting medium so as to facilitate liquefaction, preserve liquefied tissue constituents, and enable delivery of molecules into tissues. Determination of tissue composition in the liquefied tissue sample can be used in a variety of applications, including diagnosis or prognosis of local as well as systemic diseases, evaluating bioavailability of therapeutics in different tissues following drug administration, forensic detection of drugs-of-abuse, evaluating changes in the tissue microenvironment following exposure to a harmful agent, and various other applications. The methods, devices and systems are used to deliver one or more drugs through or into the site of the tissue to be liquefied.

La présente invention concerne des dispositifs, des procédés et des systèmes impliquant l'application d'énergie et/ou d'un milieu promouvant la liquéfaction à un tissu d'intérêt pour générer un échantillon liquéfié comprenant des constituants tissulaires pour permettre l'échantillonnage rapide de tissu, la décontamination rapide de tissu ainsi que la détection qualitative et/ou quantitative d'analytes qui peuvent faire partie de constituants tissulaires (par exemple, plusieurs types de biomolécules, de médicaments, et de microbes). La présente invention concerne en outre des compositions spécifiques dudit milieu de promotion de la liquéfaction pour faciliter la liquéfaction, préserver les constituants tissulaires liquéfiés, et permettre la délivrance de molécules dans les tissus. La détermination de la composition du tissu dans l'échantillon de tissu liquéfié peut être utilisée dans une variété d'applications, y compris le diagnostic et le pronostic de maladies locales et systémiques, l'évaluation de la biodisponibilité de produits thérapeutiques dans différents tissus après l'administration de médicaments, la détection post-mortem de stupéfiants, l'évaluation des modifications du microenvironnement tissulaire après exposition à un agent nuisible, et différentes autres applications. Les procédés, les dispositifs et les systèmes sont utilisés pour délivrer un ou plusieurs médicaments via ou dans le site du tissu à liquéfier.

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