Systems and methods for collecting diluted mononuclear cells

Details Systems and methods for collecting diluted mononuclear cells Systems and methods for collecting diluted mononuclear cells

B - Operations, Transporting

01

D

B01D 21/26 (2006.01) A61M 1/36 (2006.01) A61M 1/38 (2006.01) B04B 5/04 (2006.01) B04B 11/04 (2006.01)

Patent

CA 2294395

Systems and methods rotate a chamber (14), in which whole blood is centrifugally separated into an interface between the packed red blood cells and the plasma constituent. The interface carries platelets and mononuclear cells. The systems and methods include an interface control unit which is operative in a first state to enable removal of platelet-poor plasma (PPP) in a first container for use as a diluting liquid, and in a second state, to retain mononuclear cells (MNC) in the chamber (14), while removing platelet- rich plasma (PRP) from the chamber (14), bypassing the platelet-poor collection container, thereby maintaining its platelet-poor character, and in a third state to enable the removal of mononuclear cells (MNC) from the chamber (14) in a path (T13) that leads to a second container, where the mononuclear cells are collected.

Système et procédé consistant à mettre une chambre (14) en rotation, dans laquelle le sang entier est séparé par centrifugation en une interface entre les globules rouges concentrés et le plasma. Cette interface porte des plaquettes et des cellules mononucléaires. Ce système comprend une unité de commande d'interface servant, dans un premier état, à supprimer le plasma pauvre en plaquettes (PPP) en le transférant dans un premier réservoir afin de l'utiliser en tant que liquide diluant, dans un deuxième état, à retenir les cellules mononucléaires (MNC) dans la chambre (14), tout en retirant le plasma riche en plaquettes (PRP) de la chambre (14) en effectuant une déviation par rapport au réservoir de plasma pauvre en plaquettes, ce qui lui conserve son caractère de pauvreté en plaquettes, et, dans un troisième état, à permettre le retrait des cellules mononucléaires (MNC) de la chambre (14) dans un trajet (T13) conduisant vers un deuxième réservoir dans lequel sont recueillies ces cellules mononucléaires.

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