Systems and methods for efficient collection of single cells...

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/24 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01) C40B 20/00 (2006.01) C40B 30/00 (2006.01) C12M 1/26 (2006.01)

Patent

CA 2648778

A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.

L'invention concerne une plaque fabriquée pour permettre de placer des échantillons de cellules, de microorganismes, de protéines, d'ADN, de biomolécules et d'autres substances biologiques en des endroits ou sites particuliers sur la plaque afin d'effectuer des analyses adressables sur les échantillons. De préférence, une partie ou la totalité des sites sont construits à partir d'une matière que l'on peut enlever ou sous forme de palettes de façon à ce qu'un sous-ensemble des échantillons d'intérêt puisse être aisément isolé de la plaque pour un traitement ou une analyse supplémentaire. La plaque peut contenir des structures ou des traitements chimiques qui renforcent ou favorisent la fixation et/ou la fonction des échantillons et qui favorisent ou facilitent leur analyse. L'utilisation de la plaque permet avantageusement la sélection et le tri de cellules sur la base de phénomènes dynamiques et la formation rapide de transfectants stables.

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