Systems and methods for harvesting mononuclear cells by...

B - Operations – Transporting – 04 – B

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B04B 11/04 (2006.01) B01D 21/26 (2006.01) B04B 5/04 (2006.01) B04B 13/00 (2006.01) A61M 1/36 (2006.01)

Patent

CA 2293298

A blood separation system operates in a first mode to convey whole blood into the inlet region (48) of a blood processing chamber (38) for centrifugal separation into packed red blood cells, a plasma constituent, and an interface, which carries mononuclear cells, between the packed red blood cells and the plasma constituent. The system removes packed red blood cells and the plasma constituent from the chamber (38), while maintaining the interface within the chamber (38). The system operates in a second mode to remove the interface from the chamber (38) by conveying packed red blood cells into the inlet region (48). An outlet path (46) conveys the removed interface from the chamber (38). The outlet path (46) includes a first sensing element (OS) to locate mononuclear cells in the removed interface and provide a sensed output upon locating mononuclear cells.

Système de séparation sanguine fonctionnant dans un premier mode servant à faire pénétrer du sang entier dans la zone d'entrée (48) d'une chambre (38) de traitement sanguin afin de le séparer par centrifugation en globules rouges concentrés, en plasma et en une interface portant des cellules mononucléaires, entre les globules rouges concentrés et le plasma. Ce système supprime les globules rouges concentrés et le plasma depuis la chambre (38), tout en conservant l'interface à l'intérieur de la chambre (38). Ce système fonctionne dans un deuxième mode afin de supprimer l'interface depuis la chambre (38) en introduisant les globules rouges concentrés dans la zone d'entrée (48). Un trajet de sortie (46) transporte l'interface supprimée hors de la chambre (38). Ce trajet de sortie (46) comprend un premier élément de détection (OS) servant à localiser les cellules mononucléaires dans l'interface supprimée et à produire une sortie détectée au moment de la localisation des cellules mononucléaires.

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