Systems and methods for identifying a culture as positive...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) G06F 19/10 (2011.01) C12M 1/34 (2006.01) C12Q 1/06 (2006.01)

Patent

CA 2715569

Systems, methods, and apparatus for determining whether a culture in a vessel contains a plurality of microorganisms are provided. A normalization relative value is calculated for each respective measurement of a biological state of the culture between (i) the respective measurement and (ii) an initial biological state. For each fixed interval of time points, a derivative of the normalization relative values in the interval of time points is calculated, thereby forming a plurality of rate transformation values. For each set of rate transformation values in the plurality of rate transformation values, a measure of central tendency of the values in the set is computed, thereby forming a plurality of average relative transformation values. A determination whether the culture contains the microorganisms is made based on whether any calculated average relative transformation value exceeds a first threshold or whether an extent of growth exhibited by the culture exceeds a second threshold.

L'invention concerne des systèmes, des procédés et un appareil pour déterminer si une culture dans un récipient contient une pluralité de micro-organismes. Une valeur de normalisation relative est calculée pour chaque mesure correspondante d'un état biologique de la culture entre (i) la mesure correspondante et (ii) un état biologique initial. Pour chaque intervalle temporel fixé, un dérivé des valeurs de normalisation relatives dans l'intervalle temporel est calculé, ce qui permet de former une pluralité de valeurs de transformation de vitesse. Pour chaque ensemble de valeurs de transformation de vitesse dans ladite pluralité de valeurs de transformation de vitesse, une mesure de la tendance centrale des valeurs dans ledit ensemble est calculée, ce qui permet de former une pluralité de valeurs moyennes de transformation relatives. On détermine si la culture contient ou non les micro-organismes sur la base de savoir si une valeur quelconque moyenne de transformation relative calculée dépasse un premier seuil ou si un degré de croissance présenté par la culture dépasse un second seuil.

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