Systems and methods for multiplex analysis of pcr in real time

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2672034

The present invention provides methods and systems for real-time measurements of PCR with multiplexing capability. Certain embodiments relate to methods and systems that use fluorescently encoded superparamagnetic microspheres for the immobilization of amplification products during the PCR process, and an imaging chamber of a measurement device that is also capable of controllable thermal cycling for assisting the PCR process.

La présente invention concerne des procédés et des systèmes de mesure en temps réel de PCR (amplification en chaîne par polymérase) à capacité de multiplexage. Certains modes de réalisation ont trait à des procédés et à des systèmes qui utilisent des microsphères superparamagnétiques codées par fluorescence pour l'immobilisation de produits d'amplification lors du procédé PCR, et à une chambre d'imagerie d'un dispositif de mesure qui peut aussi effectuer un cycle contrôlable thermique pour aider le procédé PCR.

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