Systems and methods for predicting oligonucleotide melting...

Details Systems and methods for predicting oligonucleotide melting... Systems and methods for predicting oligonucleotide melting...

G - Physics

06

F

G06F 17/00 (2006.01) C12Q 1/68 (2006.01) G06F 17/10 (2006.01)

Patent

CA 2490466

Software systems and methods for predicting the melting temperature (Tm) and other characteristics of oligonucleotides, including modified oligonucleotides. Modified oligonucleotides are provided containing bases selected from unsubstituted and 3-substituted pyrazolo[3,4-d]pyrimidines and 5- substituted pyrimidines, and optionally have attached minor groove binders and reporter groups.

L'invention concerne des systèmes et des procédés logiciels de prévision de la température de fusion (Tm) et d'autres caractéristiques d'oligonucléotides, notamment d'oligonucléotides modifés. Les oligonucléotides modifiés contiennent des bases choisies parmi des pyrazolo[3,4-d] pyrimidines non substituées et substituées en position 3 et des pyrimidines substituées en position 5, et possèdent éventuellement des liants de petits sillons et des groupes rapporteurs y étant fixés.

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