Systems for tightly regulated gene expression

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2549263

The present invention relates to bacterial expression vectors. In particular, the present invention provides tightly-regulated bacterial expression vectors designed for the cloning and expression of toxic proteins, RNA, and metabolites in vivo. The present invention thus provides methods of expressing protein and RNAs that were previously not able to be expressed.

L'invention concerne des vecteurs d'expression bactérienne. Plus particulièrement, elle porte sur des vecteurs d'expression bactérienne à régulation stricte conçus pour le clonage et l'expression de protéines toxiques, d'ARN, et de métabolites in vivo. L'invention se rapporte donc à des procédés d'expression de protéines et d'ARNs qui ne pouvaient pas être exprimés auparavant.

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