Systems, illumination subsystems, and methods for increasing...

Details Systems, illumination subsystems, and methods for increasing... Systems, illumination subsystems, and methods for increasing...

G - Physics

01

N

G01N 21/21 (2006.01) G01N 21/64 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2591200

Systems, illumination subsystems, and methods for increasing fluorescence emitted by a fluorophore are provided. One system configured to measure fluorescence of particles includes an illumination subsystem configured to illuminate the particles with light having linear polarization oriented in a non-vertical direction, circular polarization, or elliptical polarization. The polarization of the light causes fluorescence emitted by the fluorophore to be brighter than fluorescence emitted by the fluorophore when illuminated with linearly polarized light oriented in a predominantly vertical direction or non- polarized light. The system also includes a detection subsystem configured to generate output signals responsive to the fluorescence emitted by the fluorophore.

L~invention concerne des systèmes, des sous-systèmes d~éclairage et des procédés pour augmenter la fluorescence émise par un fluorophore. Un système configuré pour mesurer la fluorescence de particules comprend un sous-système d~éclairage configuré pour éclairer les particules avec une lumière ayant une polarisation linéaire orientée dans une direction non verticale, une polarisation circulaire ou une polarisation elliptique. La polarisation de la lumière rend la fluorescence émise par le fluorophore plus lumineuse qu~une fluorescence émise par le fluorophore en cas d~éclairage avec une lumière polarisée linéairement orientée dans une direction principalement verticale ou une lumière non polarisée. Le système comprend également un sous-système de détection configuré pour générer des signaux de sortie en réponse à la fluorescence émise par le fluorophore.

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