Systems, storage mediums, and methods for identifying...

G - Physics – 01 – N

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G01N 15/10 (2006.01) G01N 15/14 (2006.01)

Patent

CA 2699319

Methods, storage mediums, and systems for correlating pulses generated from multiple interrogation regions in a flow cytometer to particular particles flowing through the flow cytometer are provided. Embodiments of the methods, storage mediums, and systems include configurations for calibrating a flow cytometer using a calibration particle having a unique signature to determine a time-of-flight for particles flowing through the flow cytometer. Based on the calculated time-of-flight and relative positions of interrogation regions corresponding to collectors of the flow cytometer, the methods, storage mediums, and systems may further include configurations for associating other signal pulses to particles of one or more different particle sets.

L'invention concerne des procédés, des supports de stockage et des systèmes permettant de corréler des impulsions produites par plusieurs régions d'interrogation dans un cytomètre de flux à des particules particulières qui s'écoulent dans le cytomètre de flux. Des modes de réalisation des procédés, des supports de stockage et des systèmes comprennent des configurations d'étalonnage d'un cytomètre de flux au moyen d'une particule d'étalonnage ayant une signature particulière afin de déterminer le temps de vol de particules s'écoulant dans le cytomètre de flux. D'après le temps de vol calculé et les positions relatives des régions d'interrogation correspondant aux collecteurs du cytomètre de flux, les procédés, les supports de stockage et les systèmes peuvent comprendre en outre des configurations permettant d'associer d'autres impulsions signaux aux particules d'un ou plusieurs ensembles de particules différents.

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