T-dna/protein nano-complexes for plant transformation

Details T-dna/protein nano-complexes for plant transformation T-dna/protein nano-complexes for plant transformation

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01)

Patent

CA 2741918

A DNA/protein nano-complex for transfecting eukaryotic cells, wherein the nano-complex comprises an expressible nucleotide molecule complexed with a VirD2 protein molecule, and a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex. The nucleotide molecule/VirD2 protein molecule/RecA protein molecule complexed may be further complexed to a cell penetrating peptide. The DNA/protein nano-complex can be used for delivering the expressible nucleotide molecule to target plant cells, in particular, monocot plant cells exemplified by wheat cells, corn cells, triticale cells, barley cells , and rye cells.

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