T-factor, coa-spc substantially free of proteolytic enzymes...

Details T-factor, coa-spc substantially free of proteolytic enzymes... T-factor, coa-spc substantially free of proteolytic enzymes...

A - Human Necessities

61

K

150/3, 195/33.1

A61K 39/00 (2006.01) C12N 9/00 (2006.01) C12P 1/02 (2006.01) C12Q 1/00 (2006.01) C12Q 1/25 (2006.01) G01N 33/60 (2006.01)

Patent

CA 1117879

ABSTRACT OF THE DISCLOSURE CoA-SPC Bakers' yeast extract which is substantially free of proteolytic enzymes and having a purity of at least 45 fold. The CoA-SPC having a satisfactory purity can be prepared by freezing and subsequently thawing Bakers' yeast. The resulting liquid and solid phases are separated. The solid phase is then subjected to conditions which preferentially solubilize insoluble proteinaceous materials other than the CoA-SPC which are bound to solid phase yeast material. The resulting solubilized proteinaceous materials are separated from the solid phase containing the insoluble CoA-SPC. The CoA-SPC is solubilized by contacting this solid phase with the liquid phase which was originally formed upon thawing of the yeast. The solubilized CoA-SPC is then removed. The CoA-SPC prepared in this manner contains far less extraneous proteinaceous material than does conventionally prepared CoA-SPC.

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