T7 dna polymerase

Details T7 dna polymerase T7 dna polymerase

C - Chemistry, Metallurgy

12

P

195/122, 195/1.1

C12P 19/34 (2006.01) C12N 9/12 (2006.01) C12N 9/99 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1335263

A method for producing blunt-ended double stranded DNA from a linear DNA molecule having a single stranded region, wherein the 3' end of said molecule is double stranded and has no 3' protruding termini, comprising incubating said DNA molecule with a processive DNA polymerase essentially free from naturally occurring exonuclease activity. Also provided is a method for in vitro mutagenesis of a cloned DNA fragment comprising providing a primer and a template, said primer having contiguous bases able to base-pair with contiguous bases of said template except at least one base which is unable to base-pair with said template, and extending said primer with a processive DNA polymerase having less than 500 units of exonuclease activity per mg of polymerase.

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