Tagging reagents and methods for hydroxylated compounds

G - Physics – 01 – N

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G01N 30/72 (2006.01) C07B 59/00 (2006.01) C07J 1/00 (2006.01) C07J 9/00 (2006.01) C07J 75/00 (2006.01) C40B 40/04 (2006.01) C40B 70/00 (2006.01) G01N 30/86 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2667854

In various aspects, the present teachings provide labeling reagents and sets of labeling reagents containing one or more heavy atom isotopes for the relative quantitation, absolute quantitation, or both, of hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols. In various aspects, the present teachings also provide methods for the analysis hydroxylated compounds including, but not limited to, hydroxylated ring containing compounds, steroids and sterols my MS/MS methods, in particular using mass differential tags.

Dans divers aspects, la présente invention concerne l'élaboration de réactifs de marquage et de jeux de réactifs de marquage destinés à la quantification relative, à la quantification absolue, ou les deux, de composés hydroxylés incluant, mais sans s'y limiter, des composés contenant un cycle hydroxylé, des stéroïdes et des stérols. Dans divers aspects, la présente invention concerne également des procédés destinés à l'analyse de composés hydroxylés incluant, mais sans s'y limiter, des composés contenant un cycle hydroxylé, des stéroïdes et des stérols à l'aide de procédés MS/MS.

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