C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/56 (2006.01) A01H 5/00 (2006.01) A23K 1/16 (2006.01) A23L 1/28 (2006.01) A61K 38/47 (2006.01) A61P 3/06 (2006.01) C12N 1/15 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12P 19/14 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2403486
Three novel polypeptides possessing (endo) .beta.-1,4-glucanase activity (EC 3.2.1.4) are disclosed which can degrade cellulose in plant extracts and plant materials. They are thus cellulases and can cleave .beta.-D-glucan polymers at internal (1-3, 1-4 or 1-6) bonds between adjacent glucose units. The amino acid sequence and encoding DNA sequence is given of all 3 glucanases (CEA, CEB and CEC) which were obtained from the fungal strain Talaromyces emersonii. The glucanases can be used to treat cellulose in the preparation of edible foodstuffs and animal feed.
Cette invention a trait à trois nouveaux polypeptides dotés d'une endoactivité .beta.-1,4-glucanase (EC 3.2.1.4), lesquels polypeptides sont en mesure de dégrader la cellulose dans des extraits de végétaux et des végétaux. Il s'agit de cellulases pouvant couper des polymères de .beta.-D-glycanne au niveau des liaisons internes (1-3, 1-4 ou 1-6) entre des motifs glucoses adjacents. La séquence aminoacide et la séquence d'ADN codante concernent les trois glucanases (CEA, CEB et CEC) obtenues à partir de la souche fongique Talaromyces emersonii. Il est possible d'utiliser ces glucanases pour traiter de la cellulose lors de la préparation de produits alimentaires et d'aliments pour animaux.
Daran Jean-Marc Georges
Herweijer Margareta Adriana
Teufel Daniel Paul
Van Den Hombergh Johannes Petrus Theodorus Wilhelmus
Van Der Laan Jan-Metske
Basf Se
Dsm N.v.
Osler Hoskin & Harcourt Llp
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