Tandem bridging: a novel method of reconstructing desired...

C - Chemistry – Metallurgy – 12 – N

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195/1.14

C12N 15/00 (2006.01) C12N 1/21 (2006.01) C12N 15/29 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01)

Patent

CA 1294230

ABSTRACT OF THE DISCLOSURE Recombinant vectors and methods for constructing ricin B are disclosed; The coding sequence for ricin B was cloned, disposed in suitable expression vectors and produced free of components normally accompanying this peptide. In addition, a novel means of reconstructing missing portions of coding sequence, and certain improvements in messenger RNA purification are disclosed.

570804

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