Tangential flow affinity ultrafiltration

Details Tangential flow affinity ultrafiltration Tangential flow affinity ultrafiltration

C - Chemistry, Metallurgy

07

K

530/3.26, 530/3.

C07K 1/34 (2006.01) C07K 14/805 (2006.01)

Patent

CA 1297630

ABSTRACT Biological macromolecules are separated from liquid mixtures in which they are contained, in substantially pure form, by a process involving tangential flow ultrafiltration of the liquid mixture in the presence of an affinity gel which binds selectively to the biological macromolecule to be recovered in pure form, in either the positive affinity absorption mode or the negative affinity absorption mode. Thus a mixture containing the biological macromolecule of interest, such as hemoglobin or (oxy)hemoglobin is first mixed with an affinity gel, such as agarose-ATP, which selectively binds to the hemoglobin or (oxy)hemoglobin and then the liquid is subjected to tangential flow ultrafiltration, so that all components of the mixture except the gel-bond hemoglobin pass through the filter. In a second stage, the gel-bond hemoglobin is treated with a salt solution to displace the hemoglobin from the gel, and then the mixture is passed again over a tangential flow ultrafiltration membrance, so that the pure hemoglobin is separated from the gel, and collected in filtrate. The affinity gel which remains in retentate can then be regenerated and re-used.

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